Molecular biology
Knowledge and insights about Molecular biology from the Science Hub
Articles
Having trouble when amplifying GC-rich sequences? Here are four tips on how to troubleshoot
GC-rich templates in DNA sequences have 60% or more G (guanine) or C (cytosine) bases. Amplifying GC-rich templates may require adjusting reagents, annealing temperature, and using specialized master mixes or standalone polymerases for optimal results, and in this blog article you get four tips on how to troubleshot.
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How to evaluate your qPCR and RT-qPCR results
qPCR and RT-qPCR methods are described, their usefulness and power as well as their sensitivity and vulnerability. In addition, it is explained how qPCR evaluation is accomplished by applying the “MIQE” guidelines and NEB´s “dots in boxes” quality scoring methods.
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ß-actin Control kit Yakima Yellow® - Eclipse Dark Quencher®
Kaneka Eurogentec
ß-actin Control kit Yakima Yellow® - Eclipse Dark Quencher® - 250 RXN (50 µL)
ß-2 microglobulin Control kit Yakima Yellow® - Eclipse Dark Quencher®
Kaneka Eurogentec
ß-2 microglobulin Control kit Yakima Yellow® - Eclipse Dark Quencher® - 250 RXN (50 µL)
UltraPureGold™ 200 nmol scale
Eurogentec
UltraPureGold™ 200 nmol scale - 1 dual purification
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